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School biology notes: Defence - ways of fighting infectious diseases, disease tests, new drugs

Keeping healthy - The bodies different defences against infection and helpful 'medications'

The body's ways of fighting infectious diseases, detecting and treating diseases e.g. vaccination-immunisation, drugs, antibiotics, monoclonal antibodies, disease tests

 Doc Brown's school biology revision notes: GCSE biology, IGCSE  biology, O level biology,  ~US grades 8, 9 and 10 school science courses or equivalent for ~14-16 year old students of biology

 How does our body defend itself when it becomes infected?   What are the physical and chemical methods of protection?   What is a pathogen?   What is our immune system?  What is a vaccine?  How does vaccination-immunisation protect us?

Sub-index for this page

(a) Starting with a historic note on cleanliness!

(b) What types of dangers are out there? Types of pathogens

(c) Our bodies physical and chemical defences against infectious diseases

(d) A detailed description of the body's immune system

(i) white blood cells  (ii) action of phagocytes  (iii) lymphocytes - formation of antibodies

(iv) action of memory lymphocytes  (v) production of antitoxins

(e) Vaccination-Immunisation - including MMR

(f) Drugs - introduction to medicines to treat disease

(g) Using antibiotics to kill or inhibit the growth of bacterial pathogens

(h) Using antiseptics and antivirals to combat pathogens

(i) Developing new drugs and medicines

(j) Monoclonal antibodies - production and uses

(k) Tests and methods for detecting diseases

See also Culturing microorganisms like bacteria - testing antibiotics

and for more on fighting disease see also

Keeping healthy - communicable diseases - pathogen infections

Keeping healthy - non-communicable diseases - risk factors for e.g. cancers



(a) Starting with a historic note on cleanliness

Be aware that our bodies provide a good environment for many microbes to live and multiply at our expense and can make us ill once they are inside our body.

Our bodies need to be capable of stopping most microbes from getting in and dealing with any microbes which do get in.

A simple example of how science works - cleanliness reduces the incidence of infection!

Appreciate the contribution of Semmelweiss in controlling the rate of patient infection to solving modern problems with the spread of infection in hospitals.

Semmelweis worked in Vienna General Hospital in the 1840s and witnessed large numbers of women dying after childbirth from a puerperal fever disease.

He thought that the staff of the hospital were spreading the disease via unwashed hands.

After instructing doctors and nurses to wash their hands in an antiseptic solution, the mortality rate was considerably reduced.

Although Semmelweis didn't realise it at the time, the antiseptic solution was killing the infecting bacteria.

Apparently, when he left the Vienna hospital, the practice of washing hands in the antiseptic solution was relaxed, and the death rates rose again!

With the advent of new strain of bacteria today, there is now an even greater need for emphasis on hospital hygiene than ever before - so, if on a hospital visit, PLEASE WASH YOUR HANDS in the antiseptic gel provided.


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(b) What types of dangers are there? Types of pathogens

Microorganisms that cause infectious disease are called pathogens.

Bacteria and viruses may reproduce rapidly inside the body and may produce poisons (toxins) that make us feel ill.

What is a bacteria?

Bacteria and certain protozoa are very small cells which can rapidly reproduce by cell division in your body making you feel ill by damaging your body's cells and producing toxins - poisons produced as a by-product of the bacteria's cell chemistry.

What is a virus?

Viruses are NOT cells and much smaller than bacteria, but damage the cells in which they reproduce. Viruses replicate by invading a cell and using the cell's genetic machinery to reproduce themselves ie copies of the original virus. The virus 'invaded' cell then bursts releasing lots of new viruses which go on to invade more healthy cells. The cell damage makes you feel ill as your body (temporarily) fights back to make as many good cells as it can to replace those destroyed by the virus.

Fungi are also pathogens and includes microorganisms like yeasts and moulds (so don't eat mouldy food!).

 


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(c) How do our bodies defend themselves against infectious diseases?

How do our bodies defences work to counteract pathogens?

The body has two different types of barriers to protect itself against pathogens - bacterium, virus or fungus.

Our body has physical and chemical adaptations for protecting itself against pathogens.

You may well ask, where do the pathogens come from?

They are all around us, but hopefully in very low concentration!

They can be in the air, water or in soil.

Pathogens can enter the body through an accidental cut or graze of the skin.

Bacteria and fungi can be in contaminated food and so ingested.

Bacteria and viruses, unfortunately, are readily transmitted by humans!

When we cough or sneeze we spray into the air thousands of tiny moisture droplets, which may contain pathogens like bacteria or viruses, which we inadvertently breathe in. That is why, if at all possible, you should take care to cough or sneeze into a tissue and dispose of it carefully in a bin.

 

Physical defence mechanisms of protection from pathogens

Your skin and hairs and mucous in the respiratory tract can stop a lot of the pathogen cells from entering your body.

When breathing, you cannot help taking in all sorts of fine particles of dust and microbes.

The whole of the respiratory tract from the nasal passage, down the trachea and into the lungs is covered with mucous and lined with ciliated cells. Cilia are fine hairs that can move freely at their ends.

The hairs and mucous in your nose traps dust and any other particles that might contain pathogens like bacteria, before they can get down  into the lungs.

The trachea and bronchi have ciliated epithelium - shown in the diagram below.

how respiratory system protects body ciliated cells goblet cells mucus traps dust particles cilia moves them along gcse biology igcse

In between the ciliated epithelial cells are goblet cells that secrete mucus onto the surface of all the respiratory airways.

This sticky mucus traps particles like dust or microbe pathogens and the cilia (hairs) move the mucous along..

The hair-like structure of the cilia of the ciliated cells work together and move-push the mucous up to the back of the throat where it can be swallowed.

The ciliated cells have lots of mitochondria, they have a lot of work to do!

Cells that line the trachea and bronchi have cilia. These hair-like structures can move the mucous along from the lungs up to the nasal passage and back of the throat where it can be swallowed, coughed out or blow your nose, into a tissue!

Note that smoking can damage and paralyse the cilia reducing the ciliated cell's capacity to remove harmful particles, so another reason why smokers are more susceptible to respiratory diseases.

The stomach produces strong hydrochloric acid, an acid that kills most pathogens, and a safe distance from the sensitive tissue of the mouth and tongue!

Skin in good condition acts as a very effective physical barrier against pathogens.

The outer layer of epidermis skin cells are dry and dead and pathogens cannot easily get through this layer.

gcse biology diagram strcuture of skin epidermis sebacious gland root hairs pores sweat gland blood vessels igcse biology

The skin also protects the body from physical damage and dehydration.

As well as acting as a physical barrier, your skin also has sebaceous glands that secrete antimicrobial molecules that can kill pathogens.

The sebaceous glands are an 'offshoot' of the hair shaft, out of which the hair grows.

What happens if the skin is damaged?

When a cut in the skin occurs, small fragments of cells called platelets help the blood to clot quickly to seal the wound - the seal becomes the covering scab when dry, and prevent microorganisms entering the skin tissue or blood stream.

When the platelets are exposed to air through a cut, they become 'activated' and make protein fibres called fibrin, that form a mesh over the wound, and the mesh traps platelets and red blood cells to form a clot.

Clotting also reduces blood loss.

The greater the concentration of platelets in the blood the faster the clotting process ('sealing') can occur.

Chemical protection by killing pathogens

Our eyes produce a chemical called lysozyme in tears, that kills bacterial microorganisms on the surface of the eye.

Lysozymes are enzymes that break down the cell walls of bacteria, so destroying the bacteria on the surface of the eye.

Lysozymes are found in several secretions produced by the body.

As already mentioned, your stomach contains quite concentrated strong hydrochloric acid which kills the majority of pathogenic bacteria that get well beyond the mouth - sadly not all of them at times!

The saliva produced in your mouth contains molecules that can kill some of the pathogens that enter the mouth.

 

Beyond the stomach

Not all the remaining pathogens that reach the stomach from the mouth are killed by the hydrochloric acid.

Some pathogens enter the intestines and have to compete with the 'local' bacteria for food to survive.

Your gut is full of bacteria - the gut is their natural habitat.

 

These physical and chemical defences are non-specific and can counteract a variety of types of pathogens.

 


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(d) A detailed description of the body's immune System

(d)(i) What is the immune system? Why is it needed?

Pathogens like bacteria make us feel unwell because their cellular chemistry releases toxins into our body.

The body has an immune system that that recognises and destroys invasive pathogens.

Phagocyte white blood cells can destroy pathogens by directly killing them.

Lymphocyte white blood cells produce antibodies to destroy pathogens and antitoxins to neutralise the toxic waste from pathogens.

So, the immune system 'kicks in' if pathogens do get inside your body.

The white blood cells are present throughout your body in your blood system and therefore are always at hand to defend you from invading pathogens and the do so in three main ways - see sections (i) to (v) below.

Only about 1% of blood cells are white, the majority are oxygen carrying red blood cells.

If your white blood cell count is low you are more susceptible to disease and infection, because this equates to a weakening of your immune response system

For example, HIV/AIDS weakens white cell action and hence the body's immune response to infection.

HIV/AIDS disease destroys white blood cells weaker responding immune system that allows pathogens to have a more devastating effect on the body.

They cannot make enough effective antibodies to counteract a pathogen infection.

Sufferers from HIV/AIDS have insufficient lymphocytes to recognise common infections and produce the necessary antibodies - sometimes with fatal consequences from a disease that in a healthy body would not have proved fatal.

The immune system of the body produces specific antibodies to kill a particular pathogen.

This leads to immunity from that pathogen.

In some cases, dead or inactivated pathogens stimulate antibody production - vaccination - immunisation.

If a large proportion of the population is immune to a pathogen, the spread of the pathogen is very much reduced - this is known as herd immunity, which can arise either from mass vaccination or naturally if a high percentage of the population develop natural immunity to a pathogen - in either case, lots of people have the antibodies to combat the pathogen and therefore far less people can be carriers of the pathogen.

More details on the functions of the white blood cells of the immune system

What is the function of white blood cells?

What is an antibody? What is an antigen? What is an antitoxin?

If pathogens like harmful bacteria actually get into your body your immune system responds to destroy them to defend you from their harmful effects.

The most important feature of your immune system is the function of the different types of white blood cells.

These white blood cells are travelling around the whole of your body in your bloodstream and so are always available to tackle an infection.

More of the types of white blood cells can be made to tackle any major infection, but infections may take time to be cleared up completely.

When white blood cells meet an invading pathogen (bacteria, virus etc.) they can respond in three different ways.

(d)(ii) The ingesting of pathogens by white blood cells - action of phagocytes

White cells can surround 'foreign' invasive microorganisms and break them up, effectively digesting them.

The white blood cells that do this are called phagocytes and the process is called phagocytosis.

Phagocytes are made and stored in the bone marrow - the soft tissue at the centre of bones.

When an infection happens more phagocytes are released and travel through the blood to the point where the pathogen (e.g. bacteria) has entered the body and the diagram shows what happens next.

1. The phagocyte detects the presence of pathogens and moves towards them e.g. move towards a bacterium.

2. Phagocytes have a flexible membrane that changes shape and pushes out to surround one or more of the pathogens.

3. The pathogens then, via the flexible membrane, become completely enclosed in the cytoplasm of the phagocyte cell (creating a vacuole) and can then be 'digested'.

4. The pathogen (e.g. bacterium) is killed and enzymes in the cytoplasm of the phagocyte break the pathogens down and the products absorbed into the phagocyte's cytoplasm.

Phagocytes can leave the bloodstream and squeeze through capillaries and enter tissues attacked by some invasive pathogen infection. The phagocytes move to the pathogens (or toxins) and ingest them.


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(d)(iii) Lymphocytes and production of antibodies, which destroy pathogens

All invading cells have unique molecules ('molecular structure') on their surface called antigens.

When white cells encounter a 'foreign' antigen on a pathogen they don't recognise, they produce proteins called antibodies which lock onto the antigens of the pathogen making them more susceptible to phagocytosis - described above and also inhibit the pathogen from entering your cells.

Reminder: The pathogen can be a bacteria, virus or fungus.

The white blood cells that perform this task are called B-lymphocytes and the overall process is described using the diagram below.

These cells are involved with specific immune responses which can involves various mechanisms after the lymphocytes recognise pathogens and quickly reproduce to make lots of antibodies e.g.

The antibodies can cause pathogen cells to burst - a process called lysis, often due to an enzymic action.

The antibodies can bind to pathogens and destroy them.

The antibodies coat the pathogen, sticking them together so that phagocytes can ingest them.

1. Large numbers of B-lymphocyte white blood cells (grey) are always present in the blood and they can recognise OR not recognise, different types of pathogens - bacteria and viruses.

2. All invading pathogens (green O) have unique molecules on their surface called antigens (blue -, often proteins). If the surface of the lymphocyte detects the antigens (blue) on the surface of a 'foreign' pathogen they don't recognise, a response is triggered by the lymphocyte cell.

3. The lymphocyte cell begins to produce protein molecules called antibodies (black Y).

4. The antibodies move out of the cell to 'confront' the invading pathogen and will not lock onto any other pathogen.

5. The antibodies lock onto the antigens on the surface of the pathogen (e.g. invading bacteria cell).

6. The invasive pathogen is then more easily found and destroyed by another type of white blood cell - the phagocytes, which destroy them by phagocytosis - described in section (a) above.

The antibodies often cause the pathogens to clump together making it easier for the phagocyte cells to find and ingest them by phagocytosis.

The white blood cells that detect the pathogen then divide to produce more copies (clones) of the same white blood cell, which in turn make more of the antibody.

The antibodies are produced quite rapidly and move all around the body in the bloodstream to find other similar pathogens.

If exactly the same type of pathogen enters your body again, the lymphocyte cells recognise it immediately and make lots of antibodies to counteract it.

This the basis of immunity i.e. how you become immune from a disease and this is described in detail in the next section.


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(d)(iv) The 'timeline' of memory lymphocyte action

If the same type of pathogen gets into your body again, the lymphocyte cells should recognise the danger and immediately and make lots of antibodies to counteract it - this is the basis of your immunity, how you become immune from a disease.

Memory lymphocyte white blood cells (memory cells) are also produced in the immune response to a pathogen and the harmless forms you are vaccinated with.

They stay in the body for a long time and 'remember' a specific antigen on the surface membrane of a specific pathogen. This means if you get re-infected, your body's response is much faster and more effective - you might not even notice any symptoms!

The antibodies produced are specific to that type of antigen, they will not lock onto any other type of antigen, hence they are specific to a particular pathogen.

e.g. the antibody for the measles virus is different to the antibody of chickenpox virus.

The production of antibodies by the body in recognition of foreign material is called the immune response.

One the 'blueprint' antibody is made, it is rapidly reproduced, carried round the body in the bloodstream, and lock onto the specific invasive pathogens and kill them.

The immune response mechanism of the white blood cells is the same in fighting either bacterial or viral infections.

If a person becomes infected with the same pathogen microorganism, the appropriate type of white blood cell will automatically, and quickly, produce the correct specific antibodies to kill the pathogen because of the first invasion of a particularly pathogen the person has become naturally immune to the specific infection.

This is because once the white blood cells have made an antibody in response to a particular infection, they can easily recognise the specific bacterium or virus and produce the same antibody again - see below - more on memory lymphocytes.

This immunity helps prevent the immune person becoming ill again, or at least minimises the chance of 2nd attack of the specific pathogen having any significant effect.

Memory lymphocytes are naturally produced in the immune system's response to a pathogen.

When a pathogen enters your body for the first time, the immune response is slow because there are relatively few of the B-lymphocytes around capable of making the antibody to combat a particular pathogen.

Eventually, your body will produce enough of specific antibody to overcome the infection, but in the mean time, you will display symptoms of the disease.

As well as antibodies, memory lymphocytes are also produced by your immune response to a foreign antigen of a pathogen. They stay around in the body for some time and 'remember' a specific antigen on the surface membrane of a specific pathogen.

The person is now got some immunity to respond much more quickly to a second infection.

See also section on vaccination-immunisation

If the same pathogen enters your body again there are far more white blood cells around to recognise the pathogen and produce antibodies to combat it.

In other words, the secondary response is faster and stronger than the first immune response, and, in many cases, destroys the pathogen before you exhibit any symptoms.

graph of antibody response to pathogen antigen infection vaccination memory lymphocytes antibodies gcse biology igcse

The graph above illustrates the possible sequence of events involving memory lymphocytes

The body is first exposed to the antigen.

This could be from an actual pathogen infection or from vaccination with a dead or inactive form of the pathogen.

In the body's primary response, the lymphocytes produce the antibodies to counteract the threat of the pathogen.

This takes a little time, but the specific antibodies increase steadily in concentration.

Eventually, the body overcomes the infection or stops responding to the vaccination, and the antibody concentration falls.

The memory lymphocytes retain the information to recognise the shape of the antigen if re-infection occurs.

If the body becomes infected, the memory lymphocytes immediately recognise the pathogen antigen and rapidly make lots of the specific antibodies.

Because of the memory lymphocytes, the 2nd response of your immune system is faster and stronger.

The specific antibody reaches a maximum concentration to fight the antigen.

As the infection is gradually overcome the antibody concentration falls.

See this in the next section with more on vaccination-immunisation

 

Epidemics are large scale outbreaks of an infectious communicable disease.

Mass vaccination programmes help reduce the chances of an epidemic, but, a high percentage of a population needs to be vaccinated to avoid the infection spreading rapidly - this can create 'herd immunity'.

If a large proportion of the population is immune to a pathogen, the spread of the pathogen is very much reduced - this is known as herd immunity, which can arise either from mass vaccination or naturally if a high percentage of the population develop natural immunity to a pathogen - in either case, lots of people have the antibodies to combat the pathogen and therefore far less people can be carriers of the pathogen.

 

 

(d)(v) White blood cells also help to defend against pathogens with antitoxins

Producing antitoxins, which counteract the toxins released by the pathogens.

These toxic substances are non-living toxins or pathogens.

They are waste toxins produced by the cell chemistry of the invading pathogen e.g. bacterium.

diagram showing lymphocyte white blood cells producing antitoxins neutralising toxins gcse biology igcse

You can think of these antitoxins as a sort of antibody that combines with the poisonous waste product molecules produced by e.g. by bacteria to form a harmless product - a sort of chemical 'neutralising' effect (but NOT the acid-alkali neutralisation variety!).

1. The microorganism releases toxic substances into the body e.g. tissues or blood.

2. The lymphocyte white blood cells recognise the specific toxin and produces the specific antitoxin.

3. The antitoxin combines with the toxin to produce a harmless 'neutralised' particle that can be disposed of as body waste.

These antitoxins are very specific to individual toxic chemical that remove the toxicity effect of the toxins produced by pathogen cell action.

 


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(e) How can our health be further protected from pathogens?

fighting infections with vaccinations - immunisation!

Be able to explain how the treatment of disease has changed as a result of increased understanding of the action of antibiotics and immunity.

Immunisation is the action of making a person or animal immune to infection, typically by inoculation with a vaccine.

Be able to evaluate the consequences of mutations of bacteria and viruses in relation to epidemics and pandemics - data provided.

Be able to evaluate the advantages and disadvantages of being vaccinated against a particular disease - data provided.

As already mentioned, Semmelweiss recognised the importance of hand-washing in the prevention of spreading some infectious diseases.

By insisting that doctors washed their hands before examining patients, he greatly reduced the number of deaths from infectious diseases in his hospital.

Some medicines, including painkillers, help to relieve the symptoms of infectious disease, but do not kill the pathogens.

As we have seen, our immune system of the body produces specific antibodies to kill a particular pathogen.

This leads to immunity from that pathogen.

In some cases, dead or inactivated pathogens stimulate antibody production.

If a large proportion of the population is made immune to a pathogen by vaccination-immunisation, the spread of the pathogen is very much reduced - which is what the next section is all about

Vaccination-Immunisation

If you become infected with a new ('foreign') pathogen that your immune system doesn't recognise as 'friendly', it takes your white blood cells a few days to produce the antibodies to protect you.

In the mean time you are unfortunately ill and not feeling well to a greater (fatal) or lesser (a bit poorly) degree.

Vaccination is a successful method to drastically reduce the response time of your immune system and usually prevents the onset of the disease.

People can be immunised against a disease by introducing small quantities of dead or inactive forms of the pathogen into the body (vaccination).

The process of vaccination has radically changed the way we fight disease because it is not about treatment of a disease, it is all about preventing the effects of an infection.

(c) doc b Know that vaccination is an important method of preventing infection.

What is vaccination? What is a vaccine? What is immunisation?

Vaccination protects the individual from future infections and mass scale vaccination can greatly reduce the incidence of disease.

Protection is better than cure! If you become infected with a pathogen, it takes a few days for your white cell immune system to deal with the microorganism, and you can become quite ill in a few days.

Vaccination is the process of injecting the individual with small amounts of specific harmless dead/inactive microorganisms (pathogens) which carry the antigens that cause the immune system to produce the corresponding protective antibodies - even though the pathogen is in a harmless form.

Different vaccines are required for specific pathogens e.g. flue, HPV (human papilloma virus), polio and whooping cough all have their own vaccine.

The MMR vaccine contains weakened versions of the viruses that cause measles, mumps and rubella (German measles).

As well as injection, vaccines can be taken orally or using a nasal spray.

So, vaccines automatically stimulate the white blood cells to produce antibodies that destroy the invading 'foreign' pathogens.

This makes the person immune to future infections by the microorganism ie gives the individual immunity from further attacks - the overall process is referred to as immunisation.

If the same type of pathogen, that you have been vaccinated against, enters your body, your body can respond by rapidly making the correct antibody, in the same way as if the person had previously had the disease.

(c) doc bVaccination is when the vaccine is administered to you (usually by syringe injection).

Immunisation is what happens in your body after you have the vaccination.

The vaccine stimulates your immune system so that it can recognise the disease (invasive pathogen - bacteria or virus) and protect you from future infection (i.e. you become immune to the infection).

The diagram and notes below what happens on vaccination to complete the immunisation effect.

1. You are injected by vaccination with a weakened/inactive/dead form of the pathogen - although harmless, your body will respond to the 'new' antigens detected - an immune response.

2. Your lymphocyte white blood cells recognise the pathogen as harmful and produce the antibodies to counteract 'what is perceived' as an active pathogen.

3. If the same actually active pathogen enters your body, it is quickly recognised by its antigen molecules and attacked by the specific antibodies already present and more can be made too, quite rapidly.

4. The effect of the pathogen is 'neutralised' so you don't become ill.

5. When the pathogens are combined with the antibodies they are much more susceptible to be ingested by the phagocyte white blood cells and destroyed.

MMR vaccine is used to triple protect children against measles, mumps and rubella (German measles).

The vaccine contains weak inactive versions of three viruses that cause measles, mumps and rubella.

The effects of vaccination can 'wear off' over time, and booster injections maybe necessary to increase the levels of the protective antibodies.

graph of antibody response to pathogen antigen infection vaccination memory lymphocytes antibodies gcse biology igcse

The graph above illustrates the possible sequence of events involving immunisation

The body is first vaccinated with a dead or inactive form of the pathogen.

In the body's primary response, the lymphocytes recognise the antigens and produce the specific antibodies to counteract the perceived threat of the form of the pathogen in the vaccine.

Eventually, the body stops responding to the vaccination, and the antibody concentration falls, but NOT to zero!

The memory lymphocytes retain the information to recognise the shape of the antigen if an infection of the same pathogen occurs. The body has been immunised to fight this particular pathogen.

If the body becomes re-infected with the same pathogen, the memory lymphocytes immediately recognise the pathogen antigen and rapidly make lots of the specific antibodies.

So, the 2nd response of your immune system is faster and stronger compared to the original vaccination. The immunisation has worked.

The specific antibody reaches a maximum concentration to fight the antigen.

As the infection is gradually overcome the antibody concentration falls.

 

There are arguments for and against vaccination (the 'pros and cons')

For vaccination - immunisation:

Vaccines have resulted in the large scale control of many infectious diseases that were once common and often fatal e.g. measles, mumps, polio, rubella, smallpox, tetanus, whooping cough etc.

These communicable diseases were once common in the UK but smallpox has been completely eradicated and polio infections are very rare these days (down as much as 99%)

Epidemics are less likely with mass vaccination - spread of the disease is less likely as there are fewer infected people to carry an active form of the disease - but a large percentage of the population needs to have been vaccinated - less people around to carry and pass on the pathogen.

This means people who aren't immunised, are less likely to catch the disease as there are far less people to pass it on. This situation is known as 'herd immunity'  - lots of people have the antibodies to combat the pathogen and therefore far less people can be carriers of the pathogen..

Herd immunity is defined as the resistance to the spread of a contagious disease within a population that results if a sufficiently high proportion of individuals are immune to the disease, especially through vaccination - in either case, lots of people have the antibodies to combat the pathogen and therefore far less people can be carriers of the pathogen..

Without mass vaccination an outbreak of epidemic proportions is much more likely - many more people potentially to carry and transmit the disease which can spread rapidly, particularly in densely populated areas where lots of people are in close contact.

A notes of caution on using vaccines!

(i) After the two 'shots' of the MMR vaccine as a child, your protection from measles, mumps and rubella should last you a lifetime.

(ii) Others, like the injection for protection against the tetanus bacterium and polio virus, only give you immunity for about 10 years - so, in many cases of vaccination, you need booster doses.

(iii) Due to mutations of the flue virus strains, your immunity lasts a year and you need a fresh 'flue jab' before every winter - but this is your choice, highly recommended for older people like me!

(iv) No vaccine has been developed to protect us from the common cold or the HIV virus.

 

Against vaccination - immunisation:

Immunisation programmes are not always successful - some vaccines do not always give you immunity.

So, development work goes on all the time to make more effective vaccines - especially as different strains of viruses and bacteria are constantly evolving.

There may also be side-effects in which the 'patient' has a bad reaction to a particular vaccine eg swelling, fever, seizure (serious!), but such reactions and complications are rare and the mass good effect of large scale immunisation should be balanced against the very rare negative effect - however serious this might be.

There are some concerns over using 'whole' pathogens so that the vaccine actually causes disease in the person. Therefore some vaccines only use parts of the pathogen cells which must include the antigens for the white blood cells to react to.

Producing vaccines and carrying out mass vaccination programmes can be expensive - the disease may be rare or the vaccine proves to be not that effective.

The benefits of vaccination must outweigh the development and production costs involved.

There is a very small risk involved with most medical treatments

Side-effects, usually minor, are not uncommon, BUT, without vaccination some of these diseases are fatal or have very serious non-fatal outcomes - people can die of from measles, rubella has serious consequences for pregnant women, there can be serious complications for infected people who have not been vaccinated.

Following a seaside accident - cut on knee, as an eleven year old, I collapsed unconscious after a tetanus injection at a local hospital. I was ok within half an hour BUT my parents got a bit of a shock!

Parents of young children are always given details of vaccination schedules and where appropriate, warned of side effects associated with specific vaccines.

Sadly in some countries, including in the UK, a lot misinformation has been put about on social media about the supposed ill-effects of taking the MMR (mumps, measles and rubella) vaccine e.g. causing autism. The information was not backed up by real scientific data and as a result was hundreds of thousands of young children were not vaccinated with three medical conditions with potentially serious consequences.


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(f) Drugs - introduction to medicines for treating disease

Drugs are substances that affect how the body works.

Most drugs are proven safe medicines to use, but some are potentially dangerous if misused.

Many drugs can be bought directly from a pharmacy, but others can only be obtained from a doctors prescription.

The first thing you should appreciate is the difference between 'feeling better' and being 'cured'!

If you are injured, some of your sensory nerve endings send pain messages to the brain - an unpleasant experience.

Painkillers block these nerve impulses, reducing pain sensation and making you feel better.

Some painkillers were originally derived from plants e.g. an aspirin like molecule is found in willow bark and opiates are extracted from the poppy flower.

From these naturally occurring molecules, lots of synthetic derivatives have been developed like codeine.

Some drugs like aspirin or paracetamol relieve pain and reduce discomfort i.e. reduce the symptoms, but they do not counteract the disease you are suffering from e.g. a virus giving you a headache - but pain killers are better than nothing and enable to carry on with life with less discomfort while your body's immune system fights the infection.

Such drugs do NOT cure you because they do NOT kill the pathogen causing the disease in the first place.

Lots of other drugs such as cold remedies, decongestants, analgesic pain killers etc., cannot destroy e.g. the cold or flue virus but do make you feel a lot better and help you get a better night's sleep!

Other drugs e.g. the antibiotic penicillin do kill or inhibit the growth of certain bacterial infections by interfering with the pathogen's metabolism e.g. the biochemical processes that build bacterial cell walls.

(See next section on antibiotics and drug development)

However, they are not a 'blanket cure', different types of bacteria require different types of antibiotic and the correct match is required to effect a cure.

Note that nothing is perfect in medicinal treatments!

Whether the drug is a painkiller, antibiotic, antiviral etc. people can suffer adverse effects e.g.

some people exhibit an allergic reaction to penicillin.

Some drugs are expensive and maybe need to be taken for a long time, without the outcome being wholly effective - doctors have to make some crucial judgements on what to prescribe.

Medical practitioners have to 'juggle' costs versus benefits, especially the more expensive the treatment.

There are also problems from the development of antibiotic-resistant bacteria like MRSA 'superbugs'

 


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(g) ANTIBIOTICS to kill or inhibit bacterial pathogens

See also Culturing microorganisms like bacteria - testing antiseptics and antibiotics  gcse biology revision notes

Unlike 'symptom relievers' like aspirin, antibiotics like penicillin do kill or inhibit the growth of certain bacterial infections.

However, they are not a 'blanket cure', different types of bacteria require different types of antibiotic and the correct match is required to effect a cure.

Never-the-less, the widespread use of antibiotics has greatly reduced the number of deaths from communicable diseases caused by bacteria.

Unfortunately, antibiotics do NOT destroy virus infections from e.g. flue or cold viral infections.

Virus attacks can be treated with very specialised and expensive anti-viral drugs, but since viruses reproduce in your own body cells, its difficult to avoid damage to you own healthy body cells.

Other drugs e.g. the antibiotic penicillin do kill or inhibit the growth of certain bacterial infections by interfering with the pathogen's metabolism e.g. the biochemical processes that build bacterial cell walls.

Antibiotics do not affect human cells AND they do not kill fungal, protist and viral pathogens - they only kill bacteria.

Bacteria are single-celled organisms that rapidly divide.

Most are harmless, but some are not, causing bacterial infections.

Antibiotics, including penicillin, are medicines that help to cure bacterial disease by killing infectious bacteria or inhibiting their growth inside the body, without killing your own body cells!

What is an antibiotic? How do they work?

NOTE: An antibiotic kills bacteria in the body.

BUT an antiseptic kills bacteria outside the body e.g. on the skin or disinfecting a worktop in the kitchen - do NOT ingest an antiseptic.

Antibiotics cannot be used to kill viral pathogens, which live and reproduce inside cells.

Antibiotics do NOT destroy viruses, typified by the cold and flue viruses we all suffer from.

Viruses make your own body cells reproduce the invasive virus and unfortunately anti-viral drugs may attack good cells too!

It is quite difficult, and costly, to develop and market anti-viral drugs that will only kill the virus and not your own body's healthy cells.

Antibiotics like penicillin kill or prevent the growth of harmful pathogens, they kill the bacteria but not your own healthy body cells.

Antibiotics work by inhibiting processes in bacterial cells inhibiting cell division, eventually killing them, but they do NOT affect the cells of the host organism.

Some antibiotics inhibit the building of the cell walls of bacteria, which prevents cell division - these antibiotics do not affect human cells which do not have cell walls.

Different antibiotics attack different bacteria, so it is important that specific bacterial infections should be treated with the appropriate specific antibiotics.

Doxycycline is an antibiotic to treat infection from the chlamydia bacteria.

Doxycycline works by interfering with the synthesis of important proteins inside the bacterial cells, that chlamydia need to survive.

Other antibiotics like penicillin are not as effective, hence the need to constant research and develop new antibiotics, especially more resistant strains of bacteria evolve.

The use of antibiotics has greatly reduced deaths from infectious bacterial diseases.

However, overuse and inappropriate use of antibiotics has increased the rate of development of antibiotic resistant strains of bacteria.

You need to be aware that it is difficult to develop drugs that kill viruses without also damaging the body’s tissues.

Explaining the use of antibiotics to control infection:

Antibiotics are taken internally e.g. intravenous syringe injection, or orally taken by tablet or liquid suspension.

Antibacterials to treat bacterial infections

Probably the most well known antibacterial is the antibiotic penicillin which is effective against many bacterial infections BUT NOT viruses like the common cold or flue.

An antibiotics can kill bacteria or prevent them growing and reproducing.

Many strains of bacteria, including MRSA, have developed resistance to antibiotics due to mutations, which cause stronger more resilient strains of bacteria to survive as a result of natural selection.

To prevent further resistance arising it is important to avoid over-use of antibiotics.

Knowledge of the development of resistance in bacteria is limited to the fact that pathogens mutate, producing resistant strains.

Mutations of pathogens produce new strains.

Antibiotics and vaccinations may no longer be effective against a new resistant strain of the pathogen.

The new strain will then spread rapidly because people are not immune to it and there is no effective treatment.

Can bacteria become resistant to antibiotics?

Unfortunately the answer is yes! Bacteria will sometimes quite naturally mutate into forms that are resistant to current antibiotics, so if you are infected with a new strain of bacteria, your resistance from your 'current' antibiotic is not as effective.

If an infection is treated with an antibiotic, any resistant bacteria from any mutations will survive and this means more resistant bacteria can survive and reproduce to infect other people, while the non-resistant strains will tend to be reduced.

This bacterial mutation is an example of natural selection at the individual cell level and drug companies are constantly trying to develop new antibiotics to combat the new evolving strains of harmful bacteria - but new harmful 'superbugs' are becoming more common the more we use antibiotics and new epidemics can break out!

MRSA, methicillin-resistant staphylococcus aureus causes serious wound infections (including after surgery in a hospital), can't be treated with many current antibiotics and causes serious wound infections that can be fatal to young babies or elderly people in particular.

Misuse by over-prescribing antibiotics is believed to be causing the rise of mutant resistant strains of bacteria, so doctors are being advised to avoid over-prescribing antibiotics to reduce the mutation rate and not treating mild infections with antibiotics.

Symptoms like headaches or sore throats are not a justification for being prescribed antibiotics. Unfortunately, many patients (for various reasons) are prescribed antibiotics when they are actually suffering from a viral infection.

BUT, if an antibiotic is appropriately prescribed, you should always complete the course, even if you feel a lot better, this is to maximise killing the bacterial infection and minimise the chance of passing on of the infection.

Understand that antibiotics kill individual pathogens of the non-resistant strain.

Individual resistant pathogens survive and reproduce, so the population of the resistant strain increases.

Now, antibiotics are not used to treat non-serious infections, such as mild throat infections, so that the rate of development of resistant strains is slowed down.

The development of antibiotic-resistant strains of bacteria necessitates the development of new antibiotics.

See also Culturing microorganisms like bacteria - testing antiseptics and antibiotics  gcse biology revision notes

 


(h)(i) ANTISEPTICS kill or inhibit the growth of pathogens

See also Culturing microorganisms like bacteria - testing antiseptics and antibiotics  gcse biology revision notes

Antibiotics kill pathogens in your body, antiseptics kill pathogens outside of your body e.g. on the surface of your skin or disinfecting surfaces in the kitchen.

Antiseptics are used to clean wounds by killing microorganisms or stopping them multiplying.

The use of antiseptics in hospitals and GP surgeries is vital to prevent the spread of infectious diseases like MRSA.

You should always cleanse-disinfect your hands with the facilities provided before visiting someone in hospital.

There are many commercial antiseptic cleaning substances available for your kitchen, toilets etc.

Most claim to 'kill 99% of all germs' !!!!

 

(h)(ii) ANTIVIRALS

Viral infections

It isn't just bacteria that can mutate, viruses can also evolve via new mutations.

Viruses are notable for the rapidity with which they can mutate which makes it difficult to develop new vaccines.

The reason being that changes in the virus (or bacteria) DNA leads to different gene expression in the form of different antigens, so different antibodies are needed.

The flue virus is a never ending problem and in the past pandemics (epidemics across many countries at the same time) have killed millions of people, mercifully this rarely happens these days thanks to antibiotics.

As I'm re-editing this page in 2020, the World is suffering from the Covid-19 pandemic!

Antivirals are drugs used to treat viral infections.

Most antivirals do not kill the virus but stop them reproducing.

They are not easy to develop as effective anti-virus agents because it is difficult to target the virus without damaging the host cells.

Why don't antibiotics counteract viral infections?

Antibiotics can't destroy viruses like the common cold or flue.

This is because a virus is NOT a living organism like plant or animal cells, so viruses don't have a cell wall or any of the organelles that function in living cells for life maintenance, growth and reproduction.

Antibiotics work by interfering with these life processes, but since viruses don't function like this, antibiotics don't affect viruses.

Viruses replicate in host cells by 'hijacking' their genetic machinery, but antibiotics are designed not to attack your healthy cells, so they don't affect the viral infection of your healthy cells.

 


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(i) Developing and testing new drugs and medicines

See also Products of the Chemical & Pharmaceutical Industries & impact on us (GCSE chemistry notes)

It has been known for some time that plants produce a wide range of chemicals to defend themselves against attack from e.g. insect pests or pathogen microorganisms.

Historically, currently and in the future, these substances provide a basis for developing drugs to treat human diseases.

Even some of our current medications come from knowledge of plants giving us many traditional herbal recipes e.g.

(i) The painkiller aspirin was developed from chemicals in willow plants that reduce fever and reduce pain in childbirth.

(ii) Drugs like digitalis have developed from chemicals found in foxglove plants and are used to treat heart conditions.

Many antibiotics are made from growing microorganisms (first found by 'accident'!) e.g.

(i) The famous scientist Alexander Fleming noticed in some petri dishes used for investigating bacteria, a mould had grown, but the area around the mould was free of bacteria.

He realised that the mould (in this case Penicillium notatum), was producing a chemical that killed the bacteria.

This chemical was extracted and named penicillin, and proved to be a very effective antibiotic in killing various bacterial infections.

(ii) These days pharmaceutical companies grow fungi and other microbes on a large scale and extract the antibiotic molecules in the laboratory.

 

Decisions - scientific and commercial!

Any new drug must be targeted at some specific medical condition where there is need - otherwise it would not make commercial sense to develop a new pharmaceutical product.

From a scientific point of view, many drugs are designed to inhibit part of the chemistry of a disease e.g. targeting a gene or a protein like an enzyme.

The target might be blocking the action of an enzyme or a gene with a chemical agent (drug) you can interfere with the development of a disease e.g. the anti-cancer drugs used in chemotherapy treatments to reduce the growth of tumour cells or kill them.

Studies of the genomes and resulting proteins in both plants and animals are proving useful to identify 'targets'.

You then have to find a chemical that will have an effect on the target.

There are databases of chemicals that have been screened by advanced analytical techniques which can be consulted for likely effectiveness.

The screening might not initially indicate the best molecule to 'hit the target' in a biochemical sense, but, it may provide a useful starter molecule.

You can then modify the starter molecule to produce a variety of derivative molecules, one of which might provide a more effective treatment. The derivative molecules can be quite similar, but it is a sort of 'fine tuning' of their molecular structure to increase the drug's effectiveness.

 

Today drugs are manufactured on a huge scale in the pharmaceutical industry.

Chemists can synthesis molecules based on naturally found organic compounds from plants and also lots of molecules that have never existed in nature until synthesised in a modern chemical laboratory.

Historically, most effective drugs were discovered by accident e.g. somebody by chance notices some effect of a chemical which might have a medical application.

However, these days, research is very systematic and we have an extensive database of knowledge about the structure and properties of molecules AND how diseases work.

Some drugs have been successfully designed by computer software that can construct and display molecular structure e.g. design a molecule with a shape to fit into the active site of an enzyme to inhibit its action.

See also Products of the Chemical & Pharmaceutical Industries & impact on us (GCSE chemistry notes)


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Developing a new drug - a lengthy and costly process!

The drugs developed and produced by the pharmaceutical industry are often very costly in the making for several reasons

You have to carry out a lot of research and development to find a suitable compound that performs an effective medical treatment for some condition e.g. to reduce blood pressure, kill cancer cells, slow down the development of dementia etc.

The compound must be tested, often modified and retested.

All new potentially useful drugs must be fully tested in trials including animal trials (controversial) and human trials and this all takes time and money.

Until a drug has fully passed all safety and effectiveness tests it cannot be marketed and sold to medical institutions from hospitals to high street pharmacies etc. The manufacturer must prove that any pharmaceutical product like a drug does meet all legal requirements that it does actually work and is safe to use.

The stages in the testing of a new drug-medicine is summarised below:

Stage 1 Preclinical testing (non-living animal testing):

Computer models can be used initially to simulate a human's responses to a drug and can identify possible effective drugs, but cannot possibly be as accurate as actually using cell tissue cultures or live animals.

In preclinical testing the drugs are tested on cultured human tissue cells in the laboratory.

However, these procedures cannot be used to test drugs that affect a complex body system.

e.g. a drug for controlling blood pressure must be tested on a whole live animal with its complete intact circulatory system.

Stage 2 Preclinical testing (live animal testing):

If the stage 1 tests prove satisfactory, and no potential harmful effects are detected, you can then move onto testing the drug on live animals.

This is to see whether the drug works and producing the desired medical effect (this is known as testing the efficacy of the drug).

You are also looking for potential harmful side effects, including toxicity, from using the new drug.

You are also investigating the appropriate dosage in terms of concentration/amount and frequency of administering the drug.

According to UK law, any new drug must be tested on two different live mammals, but there are objections to this on several grounds:

(i) Many people object on the grounds it is cruel and unethical to use animals in tests, but others think drug safety should override these considerations i.e. avoid the use of a potentially dangerous drug.

(ii) Animals used in testing drugs are not quite the same as humans. Could their biological differences give us false results in terms of the efficacy of the drug when used on humans?

Stage 3 Clinical testing on humans:

If the drug has passed all the preclinical tests, you can then test it on human volunteers in what is called a clinical trial - which is just as complicated as any research laboratory testing.

Initially, the drug is tested on healthy volunteers - this is the only way to find out if there are any harmful side effects on a healthy body working normally.

Initially it is unsuitable to test the drug on sick people who are likely to be more vulnerable to side effects.

At first very low doses of the drug are administered to healthy people and then the dose is gradually increased and the participants medical state closely monitored.

If the results of the tests on healthy individuals are good and meet any health and safety criteria, the drug can then be tested on patients suffering from the illness-disease the drug is designed to combat.

From these tests the optimum dose is found - that is the dose that is most effective with the fewest side effects.

The safety and effectiveness of the new drug must be thoroughly checked out.

This takes some time, human drug trials may last for months or even years.

Sometimes this is due to the long term progression of a disease e.g. cancer and the time taken for the treatment to be shown to be effective.

It might also be a long time before the symptoms of side effects show up.

We now get into the practice of how to get statistically valid results from real patients.

To test the effectiveness of a drug a group of patients are randomly selected into two groups.

One group is given the new trial drug and the other group, the control group, a placebo - a substance that looks like the drug being tested, but has no effect - it can be just a sugar pill.

The control group (placebo group) of a clinical trial should be similar to the people actually being treated with the trial drug e.g. of similar age and gender.

Using a placebo ensures the doctors can see the real difference the drug is having on the patient's condition. This also allows for where the patient does expect an improvement in their medical condition and might actually feel better - even though unknowingly, nothing has been taken to improve the medical situation!

In some trials on seriously ill patients, placebos are not used - it would be unethical not to allow all patients the same chance of benefiting from the new drug.

In other trials doctors might test new drugs against the best existing treatment instead of testing against a placebo.

Note that that clinical trials must be blind, meaning, the patient in the trial doesn't know whether they are getting the new drug or a placebo, but the doctors do know who has the trialled drug or the placebo.

Sometimes the clinical trials are double-blind where neither the patients nor the doctors know who has the drug or placebo until all the results have been gathered and analysed. This ensures the doctors administering, monitoring and analysing the drug trial are not subconsciously influenced by their knowledge of the patients.

Before any drug is approved for use in our healthcare systems, the results of drug testing trials must be peer reviewed by other equally qualified medical practitioners.

This is essential to avoid false or biased claims of the new drug's performance in real patients.

Drug trials can also 'open-label' where the doctor and the patient are aware of who is receiving the drug. This can be used when comparing the effectiveness of two similar drugs being trialled.

Peer reviewers must check the validity of the drug trial e.g. has it been correctly designed and rigorously carried out to the highest scientific standards.

Finally, the drug can only be approved for patients of the general public after permission is granted by the appropriate medical agency if all health and safety criteria are met. The rules are strict to ensure the drugs are as effective and safe to use as possible.

Thalidomide: A tragic classic case of insufficient testing

Thalidomide is a drug that was developed as a sleeping pill in the 1950s and was tested for its effectiveness, but only as a sleeping pill.

Later it was also found to be effective in relieving morning sickness in pregnant women.

However, the drug thalidomide had not been tested for use in pregnant women, in particular it was not tested for relieving morning sickness.

Also, it was not known that the drug could pass through the placenta and into the foetus (fetus), where unfortunately, it caused abnormal limb development.

Thousands of babies were affected and about half survived with missing limbs or malformed limbs.

Around 10 000 babies were affected and only half of them survived.

It was only after many babies born to mothers who took the drug were born with severe limb abnormalities that the drug was then banned for this use.

As a result, drug testing has become much more rigorous in an attempt to reduce the incidence of serious side-effects from newly developed drugs..

More recently, thalidomide has been used successfully in the treatment of leprosy and other diseases including some cancers.

 


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(j) Monoclonal antibodies - production and uses

You need to have read about antibodies before studying this section.

How do you make monoclonal antibodies?

As we have seen, antibodies are produced by the type of white blood cell called B-lymphocytes. It is proving useful to medicine to produce lots of a specific antibody from multiple clones of a single white blood cell. The antibodies will be identical and only target one specific antigen protein molecule. Unfortunately, lymphocyte cells do not divide easily, but tumour cells can be readily cultured to undergo rapid cell division.

The process starts by (i) injecting a mouse with a specific antigen, this stimulates the production of antibodies against the antigen and then extracting the B-lymphocytes produced.

(ii) Culturing fast dividing tumour cells called myeloma cells.

(iii) You then fuse a mouse B-lymphocyte with a tumour cell to create a 'hybrid' cell called a hybridoma cell - which can be cloned to make lots of identical cells. It is these cells that produce identical monoclonal antibodies, which can be collected and purified for research or direct medical use.

If possible, you can produce monoclonal antibodies that bind to anything you want e.g. an antigen that is only found on the surface of a one specific type of cell.

Because monoclonal antibodies only bind to a specific antigen molecule, you can therefore target a specific cell and destroy it (e.g. a cancer cell) or 'neutralise' a chemical in the body to inhibit its poisonous action.


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Uses of monoclonal antibodies

1. Treating diseases using monoclonal antibody techniques

As we have seen, different cells in the body have different antigen molecules on their surface, which gives them a unique molecular signature.

This means you can make monoclonal antibodies that will bind to ('target') specific cells with that specific antigen.

Cancer cells have antigens on their cell membranes that you do not find on normal healthy body cells and they are known as tumour markers.

In the laboratory you can culture cells to produce monoclonal antibodies (see above) that will bind to these tumour marker antigens, but the real trick is other things you can do with the monoclonal antibody e.g. diagnose and treat cancer.

An anticancer drug-agent can be attached to the monoclonal antibody - see the diagram below.

The anti-cancer agent might be a toxic drug or radioactive substance (radioisotope) or any chemical that inhibits the growth and division of cancer cells.

Any toxic effect will only kill the cancer cells, not the healthy non-cancerous cells, because the anti-cancer agent is only attached to the cancer cell antibody, which itself, will only attach itself to the cancer cells - that's the way the antigen-antibody mechanism works.

Advantages and problems with using monoclonal antibodies to treat disease

Despite the wonderful advantages of applying monoclonal antibodies to medical treatments, there are the 'usual' pros and cons.

In other cancer treatments e.g. chemotherapy and radiotherapy you inadvertently damage neighbouring healthy cells as well as killing the cancer cells because of the high energy of the radiation (often gamma radiation). This doesn't happen with monoclonal antibody drug cancer treatment where the side effects are much less and healthy cells are not damaged.

Unfortunately, monoclonal antibodies do cause more side effects than expected.

Symptoms exhibited include breathlessness, fever, itchy rashes, head aches, low blood pressure and nausea and vomiting.

These side effects have limited the use of monoclonal antibody drug treatments.

 

2. Tests for tracing and measuring specific substances to help in medical diagnosis

e.g. monoclonal antibody applications include ...

(a) Binding them to a specific hormone or other molecule in the blood to measure the concentration ('level' of a chemical).

(b) Testing blood samples for the presence of specific pathogens.

(c) Tracing and locating specific molecules on cell or tissue.

You first make monoclonal antibodies that bind to the specific molecule X you are investigating.

The monoclonal antibodies are then reacted chemically to bind with a fluorescent dye molecule to facilitate an analysis.

If the molecule X is present in your analysis sample, the monoclonal antibody will attach itself to it.

Therefore the presence, location and concentration of molecule X can be obtained using uv light to cause a fluorescent effect.

(d) Testing for cancer

You first make the specific antibody that will bind to the cancer cells, but this antibody is labelled with a radioisotope.

The radioactive labelled antibody is fed into the patient through a drip into the bloodstream and carried all the way around the body.

When the antibody encounters a cancer cell it will bind to it because it recognises the antigen of the cancer cell (the tumour marker).

The radiation emitted from the radioactive tracer is monitored by a special camera (linked to a computer and screen) and where the cancer cells are concentrated, the emitted radiation will be the greatest - this will show up as a bright 'hot spot' on the screen.

Therefore doctors can see exactly where the cancer is, the size of the tumour and, from previous scans, whether the cancer is spreading e.g. secondary cancers from prostate cancer i.e. cancer spreading out of the prostate gland.

See also using monoclonal antibodies to treat cancer in section 1.

(e) Using monoclonal antibodies to find blood clots

Blood clots form when proteins in the blood join together to form a solid mesh that restricts `blood flow.

You can make monoclonal antibodies, labelled with a radioactive tracer, that bind to these particular proteins.

After injection of these monoclonal antibodies into the bloodstream, a special camera (linked to a computer and screen) can pick out a where the blood clot is where there is a high concentration of the radioisotope - shown by a bright 'hot spot' on the screen from the nuclear radiation emitted by the radioisotope.

Blood clots are very potentially dangerous and this technique is able to detect them and allow the doctor to remove them before the patient comes to harm.

 

3. Pregnancy testing using monoclonal antibodies

Monoclonal antibodies are used in a pregnancy test strip/stick which can detect the HCG hormone which is only present in the urine of pregnant women.

The science behind the test is illustrated in the diagram below.

1. You wee onto the end of the strip or dip it into a collected sample of urine - the method is up to you!

2. The reaction zone is impregnated with the HCG antibody which has been modified with an enzyme (e) to facilitate a colour effect if HCG hormone is present.

As the urine diffuses up the strip this 1st antibody combines with any HCG hormone in the urine and continues moving along the strip.

3. In the test zone the HCG combination encounters and attaches itself to a 2nd, but immobile antibody.

If the HCG hormone is present in the urine the enzyme triggers a chemical reaction to give a colour change e.g. the appearance of blue colour would signify a positive pregnancy test.

If the HCG hormone is not present, no colour change is seen, indicating a negative pregnancy result

4. The control zone is to check that the strip is working correctly, irrespective of a positive result.

As the urine diffuses further up the strip it carries along some of the first HCG antibody (with enzyme e) that has not combined with the HCG hormone. It then encounters an immobile version of the 2nd antibody which already has the HCG hormone attached to it

If the pregnancy stick is behaving correctly, you should get the same colour change whether it is a positive or negative pregnancy test result.

 

Note: You can impregnate the strip with different antibodies to test for the presence of other substances in the urine e.g. the antigens on other pathogens.

 


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(k) Tests and methods for detecting diseases - in humans or other animal organisms

See also Plant diseases and defences against pathogens and pests  gcse biology revision notes

Observations

Often symptoms of some disease/infection are quite plain to see in us humans and other animals e.g.

we experience a higher than normal temperature due to some fever condition, a headache or a spotty rash.

But, what you see, feel or measure with a thermometer, might not be enough to properly identify the infection causing the disease.

And, particular problems arise if ...

(i) the symptoms are common to several diseases,

(ii) or the symptoms are uncommon.

Therefore it is sometimes necessary to turn to laboratory analysis of some kind e.g. blood tests or tissue cell tests.

Laboratory techniques

Samples of body fluids e.g. blood, faeces or tissue from the diseased organism can obtained and visually examined or analysed in various ways e.g.

(a) Blood counts

The relative numbers of red blood cells or white blood cells can be important symptoms and help diagnose medical conditions.

A complete blood count is a blood test used to evaluate your overall health and detect a wide range of disorders, including anaemia, infection and leukemia.

If any of the measured concentration of red blood cells, white blood cells (of the immune system) and platelets is abnormal, further investigation would be requires.

(b) Urine analysis

Urine analysis can detect urinal infections, kidney or liver disease and diabetes (the latter is indicated by too much glucose in urine - you can actually do a simple dip stick test).

(c) Detailed visual microscopic examination of cells

Certain diseases can be detected by examining tissue cells under an optical microscope to look for abnormalities.

Cells of abnormal shape indicate the presence of some disease.

Microorganisms such as bacteria can detected and identified by their appearance.

You can stain the samples on the microscope slide to help show up clearer any specific cell or tissue abnormalities or pathogens - the dye can latch onto and become concentrated on particular structures.

(d) Reproducing the pathogen for a more detailed analysis

If the pathogen sample is too small, it can be added to a growth medium to multiply and give a better sample to analyse - either microscopic examination for identification or from DNA analysis (see below).

See Culturing microorganisms like bacteria for more details of the aseptic techniques - to avoid contamination by other microorganisms, therefore avoid identifying the wrong pathogen.

You can also test the pathogen with a selection of antimicrobial compounds to see what kills it - this can help identify the pathogen and what treatment is most likely to be the most effective treatment.

(e) Genetic analysis - DNA sequencing

The isolated suspect microorganism sample can be subjected to DNA analysis.

The genetic profile can be matched against a database of pathogen genomes.

Laboratory tests can identify a specific pathogen by adding sections of DNA known to be complementary to the pathogens DNA.

If the added DNA strands bind to the pathogen's DNA, it means that specific pathogen is present, thereby allowing identification.

 

See also Plant diseases and defences against pathogens and pests


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General HUMAN BIOLOGY revision notes

See also cell biology index above

Introduction to the organisation of cells => tissues => organs => organ systems (e.g. in humans)

Examples of surfaces for the exchange of substances in animal organisms   gcse biology revision notes

See also Enzymes - section on digestion and synthesis  gcse biology revision notes

The human circulatory system - heart, lungs, blood, blood vessels, causes/treatment of cardiovascular disease

Homeostasis - introduction to how it functions (negative feedback systems explained)  gcse biology revision notes

Homeostasis - control of blood sugar level - insulin and diabetes  gcse biology revision notes

Homeostasis - osmoregulation, ADH, water control, urea and ion concentrations and kidney function, dialysis

Homeostasis - thermoregulation, control of temperature  gcse biology revision notes

The brain - what the different parts do and the dangers if damaged gcse biology revision notes

An introduction to the nervous system including the reflex arc  gcse biology revision notes

Hormone systems - Introduction to the endocrine system - adrenaline & thyroxine hormones  gcse biology revision

Hormone systems - menstrual cycle, contraception, fertility treatments  gcse biology revision notes

Respiration - aerobic and anaerobic in plants and animals.  gcse biology revision notes

Keeping healthy - communicable diseases - pathogen infections   gcse biology revision notes

Keeping healthy - non-communicable diseases - risk factors for e.g. cancers   gcse biology revision notes

Keeping healthy - diet and exercise  gcse biology revision notes

Keeping healthy - defence against pathogens, infectious diseases, vaccination, drugs, monoclonal antibodies

See also Culturing microorganisms like bacteria - testing antibiotics/antiseptics  gcse biology revision

Food tests for reducing sugars, starch, proteins and lipids  gcse biology revision notes

The eye - structure and function - correction of vision defects  gcse biology revision notes

Optics - lens types (convex, concave, uses), experiments, ray diagrams, correction of eye defects (gcse physics)


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