DNA, RNA and Protein Synthesis
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This page will help you answer questions such as ...
What is a nucleotide? What is its structure?
What is the structure of DNA? Why is it classed as a polymer?
How does DNA code for amino acids and hence proteins?
What is the function of DNA? How are proteins synthesised?
What is RNA (mRNA)? What is a triplet code?
The structure of nucleotides and DNA - deoxyribonucleic acid
DNA (deoxyribonucleic acid) is a large molecule essential for life and cell replication and is another example of a natural polymer. Its structure was worked out in the 1950s, notably by the Nobel Prize winners Crick and Watson, though several other notable scientists made important contributions.
DNA molecules hold all of an organism's genetic material, that is all the chemical instructions for individual cells and complex organisms to grow and develop.
The DNA is organised into large coiled up sections called chromosomes, and within the chromosomal DNA there are shorter sections called genes.
DNA encodes genetic instructions for the development and functioning of living organisms and viruses e.g. every protein molecule needed by a living organism down to individual cell level is synthesised by other molecules reading the genetic code and combining the right amino acids in the right order.
Proteins are polymers of amino acids. DNA is a polymer of nucleotides.
Every protein has a specific structure for a particular function including enzymes, and most be encoded in DNA.
The structure of DNA
Most DNA molecules consist of two polymer chains, made from four different monomers called nucleotides, connected together in the form of a double helix. Unlike man-made poly(ethene), from the monomer ethene etc. DNA is a naturally occurring polymer - long molecular chains of joined up monomer (single) molecules. The nucleotide is the small basic molecular unit - the monomer from which the polymer is formed.
Nucleotides form the building blocks of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid). An individual nucleotide consists of three molecular bits combined together - the same phosphate group, a variable base (adenine, cytosine, guanine or thymine), and the same pentose sugar (pentose just means having a ring of 5 atoms). The phosphate group and base are attached to the sugar (see left diagram of a single nucleotide).
The DNA (and RNA) polymer chain is formed by a large number of phosphate-sugar linkages. The base is a sort 'branch' off the main chain, but this helps it to intermolecular bond with a base of another opposite strand of DNA.
The result is full DNA molecule consists of two 'molecular' strands coiled together to form a double helix, but how is this helix held together?
The two polymer strands of DNA are cross-linked by a series of complementary base pairs joined together by weak bonds - cross links (base-pairing bonds shown here as on the diagram):
There are four bases in DNA holding the structure together and the same two bases are always paired together - known as complementary base pairing.
Adenine (A) with thymine (T) AT, and cytosine (C) with guanine (G) CG.
The double helix structure is shown in the diagram above on the right, illustrating how the DNA is held together by the cross-linking hydrogen bonds between the bases to hold together the double helix together.
A short section of DNA is illustrated in more detail below.
A more detailed diagram of a very short section of a double-helix DNA molecule showing the two different base pairings holding the two molecular strands together.
DNA code, genes and the triplet code
As already mentioned, DNA polymer molecules contain the genetic codes that determine which proteins are synthesised.
The order of bases in a gene of the DNA determines the order of amino acids combined in the protein.
Adenine A, thymine T, cytosine C, guanine G
The formation of mRNA and the actual synthesis of proteins in cytoplasmic ribosomes
DNA is found in a cell's nucleus and cannot move from it through the nucleus membrane because of the size of the DNA polymer molecules.
Therefore there must be a means of getting the genetic information from the nucleus to the ribosomes in the cytoplasm where the proteins are synthesised.
This is achieved using a molecule called messenger ribonucleic acid (mRNA, a type of RNA).
mRNA is shorter than DNA and a single strand molecule, but still another polymer of nucleotides.
As illustrated above, the DNA contains the gene's triple coding system for the amino acids to needed to be combined to form a specific protein - with specific molecular properties to perform a particular chemical function in an organism.
In the nucleus, using enzymes, the two strands of the DNA double helix unzip and become a template for the production of mRNA.
By pairing up the complementary bases (on the DNA and nucleotide), the correct sequential nucleotides in the nucleus are brought together to form a complementary strand of mRNA, a step in the process called transcription (diagram on right).
The smaller mRNA molecule can now migrate out of the cell nucleus into the cytoplasm and attach themselves to a ribosome (the protein 'factory'!).
In the cytoplasmic ribosomes, the mRNA now itself acts as a template of triplet codes for amino acids to be joined together in the correct sequence for a specific protein.
In order for this to happen, the amino acids in the cytoplasm are drawn into the ribosome complex and assembled in order to match the complementary triplet codes.
The amino acids are then joined together, by enzymes, in the correct order to make a particular protein.
This production of the protein, dictated by the complementary triplet codes on the mRNA, is called the translation stage.
The above diagram shows translation in more detail, including the role of another type of RNA - transfer ribonucleic acid (tRNA) which brings the amino acids together onto the mRNA.
SUMMARY of protein synthesis
So, to summarise, you start with DNA in the nucleus, then to complementary mRNA in the nucleus (transcription stage), mRNA moves into the cytoplasm and then the amino acids are joined together in the ribosomes via the complementary triplet codes (translation stage). The diagram 'sketch' below also 'attempts' to summarise what is actually a very complicated process!
SUMMARY OF DNA REPLICATION
A more detailed diagram of the base sequence replication using the DNA template.
1. The DNA double helix molecules splits in two, and the two strands then act as templates.
2. Freely moving nucleotides can be matched up to form the weak bonds between the complimentary base pairs.
3. Two identical strands of DNA produced, both identical in their original sequence of bases.
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